2019 Fiscal Year Final Research Report
Development of sensitive multi-colour detection using the protein-protein interaction assay, FlimPIA
| Project/Area Number |
17K06920
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Yuki Ohmuro (松山有紀) 東京工業大学, 科学技術創成研究院, 助教 (30571088)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | タンパク質間相互作用 / 発光酵素 |
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| Outline of Final Research Achievements |
Protein-prottein interaction assay is a key technology to understanid life phenomena, make clear causes of diseases and create biosensors for detecting pathogenic bacterium and viruses.Therefore, Wide usages and user-friendly procedures are desireble for protein-protein interaction assay.
In this study, we improved the protein-protein interaction assay, FlimPIA developed previously, and FlimPIA became a significantly practical method.
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| Free Research Field |
タンパク質工学
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| Academic Significance and Societal Importance of the Research Achievements |
FlimPIAにおけるプローブの熱安定性の向上：FlimPIAにおけるプローブの熱安定性を向上させることで、測定値の安定性が低く、またプローブの保存条件がやや厳しいという短所をなくす。
最小レポータータンパク質を利用したタンパク質間相互作用検出系の構築：ホタル由来発光酵素よりも小さなレポータータンパク質を利用することで、立体障害が小さく、プローブ作製が容易なタンパク質間相互作用検出系を構築する。
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