Analysis of transgenic male infertile mice using ART

2020 Fiscal Year Final Research Report

• Project/Area Number: 17K07139
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Kumamoto University
• Principal Investigator: takeda naoki 熊本大学, 生命資源研究・支援センター, 助教 (90304998)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: 精子 / 遺伝子改変マウス / 疾患モデル

Outline of Final Research Achievements

A disease model mice were developed by modifying protamine genes, which are causative factors of infertility disease.
Prm1 KO mouse is showed asthenozoospermia in heterozygous, and was infertile due to poor motility.However, Prm2 KO is became asthenozoospermia-like and infertile in homozygous.
These results suggest that abnormal expression of protamine genes were the direct causes of infertility, and that there is a difference in function between Prm1 and Prm2.

Free Research Field

発生生物学

Academic Significance and Societal Importance of the Research Achievements

不妊は大きな社会問題となっているが、生殖に関わるが故にその治療法や原因の解明には大きな制限がある。本研究では不妊の原因遺伝子と目されるプロタミンに着目し雄性不妊をきたす遺伝子改変疾患モデルマウスを作製し解析した。ここで習得したモデルマウス作製法や解析手法は他の不妊原因遺伝子にも応用が可能であり、新たに開発した不妊疾患モデルマウスを用いることで、新規ART(生殖補助医療)の有効性や安全性を試験できる環境を構築できるであろう。