2020 Fiscal Year Final Research Report
A novel strategy of selective gene delivery by using a uniform magnetic field
| Project/Area Number |
17K07147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | National Defense Medical College |
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Principal Investigator |
Dateki Minori 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究施設、病院並びに防衛, 生化学, 助教 (00415879)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Keywords | 遺伝子導入手法 |
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| Outline of Final Research Achievements |
We tried to establish a novel procedure for uniform magnetic field-dependent enhancement of transfection efficiency. We examined effect of a 1.5 mT uniform magnetic field on cellular reactive oxygen species (ROS) level and transfection efficiency mediated by a ROS-sensitive transfection carrier. Our experimental results revealed that a 1.5 mT uniform magnetic field transiently decreased cellular ROS level and strongly enhanced transfection efficiency mediated by polyethylenimine (PEI). The uniform magnetic field-dependent enhancement of PEI-mediated in vivo transfection was confirmed in the livers of mice. Local intensification of a uniform magnetic field in a culture dish resulted in selective gene delivery into cells on the target area. Although further examination and improvement are necessary for this procedure, our findings provide a novel option for spatial control of gene delivery.
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| Free Research Field |
遺伝子操作
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| Academic Significance and Societal Importance of the Research Achievements |
動物体内への遺伝子の導入手法にはウィルスベクターや非ウィルス性のカチオン性高分子等が広く利用されており臓器によっては高効率での導入が可能である。非ウィルス性のカチオン性高分子等による遺伝子導入に関しては、その効率を光により制御し任意の部位への遺伝子導入効率の増強、抑制が可能なケースも報告されている。これら光による遺伝子導入効率の制御に関しては有用性が認められるものの、生体組織深部への光の作用は限定的であり依然として技術的な困難を伴う。本研究は生体深部に対しても比較的容易に作用する磁場を利用し局所的な遺伝子導入効率の制御を行う手法を確立する目的で実施した。
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