Placement of chromosomes in fission yeast

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K07249
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: Chikashige Yuji 国立研究開発法人情報通信研究機構, 未来ICT研究所フロンティア創造総合研究室, 研究マネージャー (60359081)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 細胞核 / 染色体

Outline of Final Research Achievements

It is thought that the arrangement of the chromosomes in the interphase nucleus have the key role on the chromosome functions such as transcription, replication, and repair. To examine how the chromosomes arrange in nucleus of the fission yeast, we measured the distance of each chromosome region and the nuclear membrane with fluorescent microscopy using fission yeast strains, the whole chromosomes of which are made visible by lacO/ lacI-GFP in each 80-100kb and made the arrangement map of all chromosomes in the fission yeast nucleus. As a result, characteristic chromosome arrangements were found such as the region more than 500kb localizes to the vicinity of nuclear membrane, and the region where localization at near the nuclear membrane and at the interior part of nucleus are repeated every 100kb-200kb.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

分裂酵母は、比較的少ない遺伝子数や染色体数を持ちながら、ヘテロクロマチンや動原体構造、細胞周期制御など、真核生物が有するさまざまな特徴を備えたモデル生物として、広く用いられている。本研究課題においてはじめて作成した、分裂酵母の全ゲノムにわたる染色体領域の核内配置マップは、分裂酵母を用いて、複製や転写などの染色体機能とその核内配置の関係について解析を行う上で、重要なベースデータを与えるものである。