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2019 Fiscal Year Final Research Report

Regulation of mRNA metabolism by the transcription-coupled RNA methyltransferase PCIF1

Research Project

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Project/Area Number 17K07282
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Molecular biology
Research InstitutionUniversity of Toyama

Principal Investigator

Hirose Yutaka  富山大学, 学術研究部薬学・和漢系, 准教授 (00218851)

Project Period (FY) 2017-04-01 – 2020-03-31
Keywords遺伝子発現調節 / mRNA生合成 / 転写 / RNA化学修飾 / mRNAキャップ / RNAポリメラーゼII
Outline of Final Research Achievements

I identified PCIF1, a factor that interacts with the phosphorylated C-terminal domain of RNA polymerase II (Pol II), as cap-specific adenosine methyltransferase responsible for N6-methylation of m6Am at the 5’ end of Pol II-transcripts in collaboration with groups in the University of Tokyo and Riken. I also identified novel proteins interacting with PCIF1 in human cells by coimmunoprecipitation and MS analysis.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

脊椎動物RNAの5’末端に存在するm6Am修飾を触媒する新規酵素を明らかにした本研究は、mRNA化学修飾を介する遺伝子発現制御機構の解明に繋がる。さらにこの化学修飾が、ウイルスや細菌由来のRNAと自己のRNAを識別するためのマークとして機能し、生体防御に重要な役割を果たす可能性が考えられる。従って本研究の成果は、自然免疫応答の新たな機構解明や抗ウイルス薬などの創薬への応用波及効果が期待される。

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Published: 2021-02-19  

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