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2019 Fiscal Year Final Research Report

Dimerization-induced signal generation of GPCR: Single molecule observation in live cells

Research Project

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Project/Area Number 17K07333
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionKyoto University

Principal Investigator

Kasai Rinshi  京都大学, ウイルス・再生医科学研究所, 助教 (20447949)

Project Period (FY) 2017-04-01 – 2020-03-31
Keywords細胞内1分子計測 / Gタンパク質共役型受容体 / ダイマー形成
Outline of Final Research Achievements

G-protein coupled receptors (GPCRs) constitute the largest receptor family. Recently, it has been found that some species of class A-GPCRs form transient dimers in the plasma membrane. However, the function and significance of dimerization of GPCR remain unclear.
By employing dual-color single fluorescent-molecule observation technique in live cells, we succeeded in directly observing trimeric G-protein recruitment to both GPCR monomer and dimer at a 30 Hz. We also found that inverse agonists inhibit G-protein recruitment only to dimer, suggesting that the transient dimer generates the constitutive activity of GPCR (a weak signaling activity that does not depend on ligand binding). Moreover, it was also found that dimerization of GPCR itself has a signaling activity because cytosolic calcium concentration was elevated by artificially inducing GPCR dimerization. These results suggest that both dimerization and ligand binding cooperate to generate biological signals in live cells.

Free Research Field

生物物理学

Academic Significance and Societal Importance of the Research Achievements

本研究によって、長年未解明であった、Gタンパク質共役型受容体(GPCR)の一過的なダイマー形成の意義の一端が明らかになった。すなわち、一過的なダイマーを形成することで、リガンド結合に依存しないGPCRの弱いシグナル活性を生じること、ダイマー形成が安定化することで、シグナルを生じること、また、リガンド結合とダイマー形成が組み合わさることでシグナル生成を行うらしいことがはじめて明らかになった。
本発見によって、GPCRのシグナル制御に関連する、創薬等に新しい概念がもたらされると考えられる。

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Published: 2021-02-19  

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