2019 Fiscal Year Final Research Report
Finding the central core of the Golgi apparatus
| Project/Area Number |
17K07393
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Kyoto Sangyo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Keywords | ゴルジ体 / 膜タンパク質 / タンパク質局在化 / 複合体形成 |
|---|
| Outline of Final Research Achievements |
Rab1 was found to bind to the two distinct sites on GM130 and the Rab1 binding to GM130 was suggested to regulate the conformation of GM130 reading to the control of the higher-order assembly of GM130 and their association to the membrane. YIPF1 binds to proteins of 26kDa and 66kDa, YIPF2 binds to a 22kDa protein and YIPF6 binds to a 17kDa protein. Transiently expressed YIPF6 was found to be degraded by ERAD. YIPF proteins were found to be conserved in most of the eukaryotes and the 6 members of YIPF proteins were conserved widely in holozoa including animal cells. The analysis in human cells indicated that three distinct complexes are formed from two members of YIPF and localize in the upper, middle, and lower Golgi compartment. Therefore, in holozoa, YIPF proteins form complexes and differently localized at the Golgi apparatus.
|
| Free Research Field |
細胞生物学
|
| Academic Significance and Societal Importance of the Research Achievements |
当初想定していたように，ゴルジ体の膜タンパク質とゴルジ体マトリックスタンパク質が相互作用し，ゴルジ体のタネ状構造を形成している可能性はさらに高まったと考えられる。残念ながら技術的な問題からゴルジ体のタネ状構造の存在を証明するには至らなかったが，本研究で得られた知見はゴルジ体の構造形成とタンパク質局在機構を解明するための基礎として大きな役割を果たすものと思われる。
|
