2018 Fiscal Year Research-status Report
Molecular basis of immortality-to-mortality switch in the life cycle of Cnidaria
| Project/Area Number |
17K07420
|
|---|
| Research Institution | Okinawa Institute of Science and Technology Graduate University |
|---|
Principal Investigator |
ハルトゥーリン コンスタンチン 沖縄科学技術大学院大学, マリンゲノミックスユニット, 研究員 (80725458)
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Keywords | Aurelia / metamorphosis / Cnidaria / immortality |
|---|
| Outline of Annual Research Achievements |
Research project proceeds according to the research plan with minor modification.
(1) Integration of the genomic and all transcriptomic data for Aurelia life stages and tissues (P1,S1,S2,S3,S4,J1,J2) has been completed.(2) Genome browser and associated transcriptomic databases were established and accessible to the researches and public. (3) Two articles were published in connection to the project.
As we anticipated in the previous report the ChIPSeq approach (which nobody tried before in Aurelia) turned out to be difficult due to high concentration of DNAses in the somatic tissues of Aurelia (not a problem for the germline cells). Thus, instead of ChIPSeq we proceeded in FY2018 with the comparison of DNA methylation patterns between polyp and jellyfish stages which were originally planned for FY2019.
As proposed in the original research plan we successfully used PacBio sequencer to measure kinetic variation during base incorporation (dependent on methylation status of the DNA). In addition to that we used NanoPore PromethIon sequencer for direct detection of DNA modification patterns. Data analysis is in progress and we plan to submit our new data for publication within FY2019.
Despite of the initial difficulties with ChIPSeq we will proceed further with this approach as the data is important for comparison with Nematostella polyp satge.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
To my mind the project proceeds successfully and just with a minor deviation from the original plan. I started DNA methylation analysis (planned for FY2019) already in FY2018 because ChIPSeq approached needs more time to develop (nobody tried to use it in Aurleia before). This potential obstacle has been expected and did not surprise me. At the moment 2/3 of all proposed experiments of the project have been completed and I also hope to finally succeed with ChIPSeq experiments in FY2019.
|
| Strategy for Future Research Activity |
I plan to follow the reserach scheme originally proposed in my application. The only difference that the data collection for analysis of DNA methylation pattern has been already completed in FY2018 and the ChIPSeq part (originally I planned to complete it in FY2018) will proceed in FY2019 due to initial technical difference in the isolation of intact DNA-protein complexes due to high activity of DNAses in Aurelia tissues.
|
| Causes of Carryover |
I did not go to international conference as was planned initially. Budget for conference travel was partially used for purchase of sequencing consumables. That is the reason why 43,689 yen were transferred to FY2019.
|
-
[Journal Article] Medusozoan genomes inform the evolution of the jellyfish body plan2019
Author(s)
Khalturin K, Shinzato C, Khalturina M, Hamada M, Fujie M, Koyanagi 5, Kanda M, Goto H, Anton-Erxleben F, Toyokawa M, Toshino S, Satoh N
-
Journal Title
Nature Ecology and Evolution
Volume: 3(5)
Pages: 811-822
DOI
Peer Reviewed / Open Access / Int'l Joint Research
-
