Phylogenetic analysis and taxonomic review of Cirsium in Japan

2020 Fiscal Year Final Research Report

• Project/Area Number: 17K07524
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Chiba University
• Principal Investigator: Uehara Koichi 千葉大学, 大学院国際学術研究院, 教授 (20221799)
• Co-Investigator(Kenkyū-buntansha): 伊藤 元己 東京大学, 大学院総合文化研究科, 教授 (00193524) ; 渡辺 洋一 千葉大学, 大学院園芸学研究科, 助教 (30763651)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: アザミ属

Outline of Final Research Achievements

In this study, we performed genetic analysis and morphological trait measurement on Cirsium plants using a next-generation DNA sequencer, and examined the validity of the classification currently applied.
As a result of proceeding with analysis by the sequence of the chloroplast genome, the strain of Cirsium does not match the conventional division of section and subsection, and the strain of Cirsium from Japan is distributed to the strain of Hokkaido and south of Honshu. It was shown that it is roughly divided into two strains. In addition, as a result of comparative analysis of morphological traits among closely related species, there were cases where the species could not be identified, and in the analysis by the RAD-seq method for diploid species of Reflexae, genetic within the population. The variation was large and no significance was observed for interspecific variation. From this result, it was considered necessary to reexamine the classification of the entire genus Cirsium.

Free Research Field

多様性生物学

Academic Significance and Societal Importance of the Research Achievements

アザミ属は、形態的に多様で、これまで日本国内で１５０種以上記載されたが、分類形質の変異が大きく種同定が難しいと考えられていた。今回、アザミ属の系統分類を目的に、次世代型DNAシークエンサー等を用いた遺伝解析と、種レベル、集団レベルの形態形質の検討を行った。その結果、外部形態による従来の種の記載の段階で、分類形質について、複数のサンプルを用いた検討が不十分だった可能性が示された。また、遺伝解析の結果も従来の種や亜節の区分が不明瞭だった。今後より広い範囲で、従来の節・亜節を超えたサンプリングと詳細な遺伝解析、形態形質の検討を行う必要性が示された。