Analysis of ripening feature of indica rice cultivars based on secondary metabolites

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K07629
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Crop production science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Hiroshi Nakano 国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, グループ長 (10414814)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 水稲 / 高温 / 登熟 / バイオマーカー

Outline of Final Research Achievements

The rice cultivar Takanari, which has a high potential of ripening under high temperature condition, had lower concentrations of phenylpropanoid glycoside, such as 3',6-di-O-sinapoylsucrose, and γ-oryzanol, such as 24-methylenecycloartanyl ferulate, which are biomarkers with rice plants ripened under high temperature condition, in brown rice than the standard cultivar Koshihikari. To detect the quantitative trait loci (QTLs) for the concentrations of 3',6-di-O-sinapoylsucrose and 24-methylenecycloartanyl ferulate, the concentrations in the reciprocal chromosome segment substitution lines (CSSLs) were examined. The QTLs for the concentrations of 3',6-di-O-sinapoylsucrose and 24-methylenecycloartanyl ferulate were detected on chromosomes 4 and 5, respectively, in reciprocal genetic backgrounds. These concentrations were increased by the Koshihikari alleles but decreased by the Takanari alleles.

Free Research Field

作物学

Academic Significance and Societal Importance of the Research Achievements

今回、水稲の高温登熟バイオマーカーのphenylpropanoid glycoside及びγ-oryzanolの合成に関与する遺伝子候補領域がそれぞれ第4及び5染色体に存在することが明らかになった。今後、これらの領域の絞り込みを進め、遺伝子を単離・同定することで、高温環境下でも登熟の優れる品種の育成に繋げることができる。また、phenylpropanoid glycoside及びγ-oryzanolは動物に対して様々な生理活性を持つ化合物として知られているため、機能性を持つ品種の育成にも繋げることができる。