Horizontal gene transfer in biofilm and evolution

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K07714
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Nakashima Nobutaka 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (70357622)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: バイオフィルム / 次世代シーケンサ

Outline of Final Research Achievements

Biofilm samples were obtained from the bottom of a pond in Tsukuba city, and the samples were divided into two. Only one sample was treated with DNase. Genomic DNA was purified from a total of 6 samples, and the obtained DNA samples were subjected to Hiseq sequencing. The sequencing results were manipulated according to the MetaWRAP pipeline.
As a result of assembling and Binning, Bins were obtained as expected. Some Bins were found to have statistically significant abundances with and without DNase treatment. Also, we examined changes in gene composition that did not depend on Binning, and revealed the existence of statistically significantly different species with and without DNase treatment.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究は、微生物の多様性獲得のメカニズム、ゲノム進化の過程を解明するにあたって大変意義深いものである。そればかりではなく、バイオフィルムの有効利用、人体や環境に悪影響を与えるバイオフィルムの効果的除去のための基盤情報となる。なお、バイオフィルム内の微生物は抗生物質や各種化学物質、加熱に対して抵抗性を示し、現状ではその除去が困難である。