Construction of organic solvent-tolerant Escherichia coli based on quinone biosynthesis

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K07731
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Toyo University
• Principal Investigator: Doukyu Noriyuki 東洋大学, 生命科学部, 教授 (60302957)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 有機溶媒 / 大腸菌 / キノン

Outline of Final Research Achievements

Biotechnological applications for the synthesis of various valuable chemicals can reduce wastes, save raw materials, and conserve energy. Enhancement of organic solvent-tolerance in microorganisms is expected to enhance the production level of valuable chemicals in the presence of organic solvent. AcrAB-TolC efflux pump plays an important role in organic solvent-tolerance in E. coli. Three homologous transcription activators MarA, SoxS, and Rob regulate a common set of genes known as the mar-sox-rob regulons such as acrAB, and tolC involved in organic solvent-tolerance in E. coli. In this study, we found that 1,4-dihydroxy-2-naphthoic acid (DHNA) increases organic solvent-tolerance in E. coli. AcrAB-TolC pump was found to be involved in the improvement of organic solvent tolerance by DHNA. In addition, it was found that homologous transcription factors MarA and SoxS are involved in the expression of AcrAB-TolC efflux pump.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

バイオ燃料や化学製品等の有用物質生産の効率化のため、微生物の有機溶媒耐性機構が注目されている。化学製品の製造プロセスにおいて一般的に使用されている有機溶媒の存在下では、有機溶媒の細胞毒性のため、生産効率が著しく低下する。このため、用いる細胞に有機溶媒耐性を付与することが重要な課題である。本研究の成果はバイオ燃料や化学製品を製造する幅広い産業分野に普及することが期待される。