2019 Fiscal Year Final Research Report
Development of compound evaluation system based on pathway analysis using proteome analysis
| Project/Area Number |
17K07783
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioorganic chemistry
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Muroi Makoto 国立研究開発法人理化学研究所, 環境資源科学研究センター, 専任研究員 (30261168)
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Keywords | 阻害剤 / 標的分子 / 代謝 / インフォマティクス |
|---|
| Outline of Final Research Achievements |
Compounds affecting cancer metabolism are expected to contribute to the elucidation of the mechanism of cancer metabolism and a discover of such compounds may leads to the creation of new anticancer drugs. A study was conducted for the purpose of constructing a metabolic inhibitor evaluation analysis system by improving ChemProteoBase, which is a drug target analysis system using proteomic analysis by 2D-DIGE. An improved system was developed to evaluate the effect of inhibitors on metabolism by analyzing the expression level of the spots relating cancer metabolism such as glycolytic enzyme proteins, and targets of novel compounds were clarified.
|
| Free Research Field |
ケミカルバイオロジー
|
| Academic Significance and Societal Importance of the Research Achievements |
様々なスクリーニングで取得された生理活性物質の標的を同定することは、抗がん剤をはじめ新しい薬の開発につながる他、その過程に関与する新しい生命現象の解析に役立つ。しかし、標的を同定することは容易ではなく、様々な手法が開発されてきた。本研究の２次元電気泳動を用いたプロテオーム解析に基づく代謝阻害剤評価解析システムを用いることによって、がんを標的とした作用未知の化合物の迅速な作用解析が行えることが期待され、薬学、応用微生物学、天然物化学の研究進展にも寄与できると考えられる。
|
