Development of fundamental technologies for elucidation of the nature of Reston ebolavirus infection

2020 Fiscal Year Final Research Report

• Project/Area Number: 17K08091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Fukushi Shuetsu 国立感染症研究所, ウイルス第一部, 室長 (80373398)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: レストンエボラウイルス / 抗体検出 / 遺伝子検出

Outline of Final Research Achievements

The purpose of this research project is to develop fundamental technologies for elucidation of the nature of Reston ebolavirus (REBOV) infection. I analyzed genome sequences of REBOV detected from cynomolgus monkeys in the Philippines and designed novel primer/probes for a real-time PCR method to detect REBOV infection. This method is evaluated using RNA samples obtained from REBOV infected monkeys. For a serological testing, I developed competitive ELISA for detecting serum anti-REBOV antibody using REBOV-NP specific monoclonal antibody. In addition, the REBOV-GP expression system was established. It might be a useful material for developing anti-GP monoclonal antibodies.

Free Research Field

ウイルス学

Academic Significance and Societal Importance of the Research Achievements

本研究により開発される抗体および遺伝子検出法は、現在、サル検疫施設において行われている検査に加え、レストンエボラウイルス感染の確定診断法として適用できる可能があり、公衆衛生対策への技術的貢献が期待できる。