Development of culture media optimizing organelle functions for preimplantation embryos

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K08134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: University of Yamanashi
• Principal Investigator: Kishigami Satoshi 山梨大学, 大学院総合研究部, 教授 (10291064)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 発生工学 / 体外培養 / 着床前胚発生 / 細胞小器官

Outline of Final Research Achievements

This research project aims to improve the in vitro culture technique of preimplantation embryos, which is essential for human infertility treatment and developmental engineering technology of experimental animals, by controlling the functions of organelles including the nucleus and mitochondria of embryos. As a result, we have obtained important findings such as overcoming mitochondrial dysfunction, improving in vitro matured egg development, glucose metabolism and developmental competency, cell signaling and chromatin formation, autophagy and developmental competency. It is expected that these new findings will contribute to the development of new culture solutions for improving the embryonic development in the near future.

Free Research Field

発生工学分野

Academic Significance and Societal Importance of the Research Achievements

哺乳類の着床前胚の体外培養技術は、ヒトの不妊治療や実験動物の発生工学技術に不可欠である。しかしながら、現在の培養液では体内環境に比べ容易に発生が停止し、その産子への成功率が限られている。本研究課題では胚の核やミトコンドリア、オートファジーを含む細胞小器官の機能制御の視点から「機能性胚培養液」の開発を行った。本研究を通じて、発生率改善につながるいくつかの重要な知見を得ることができ、今後産子率向上に向けて培養液改善につながることが期待される。