Development of derivatization procedures using reagent analogues and isotopologues for enhancing throughput of mass spectrometry-based clinical examinations

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K08250
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Tokyo University of Science
• Principal Investigator: Higashi Tatsuya 東京理科大学, 薬学部薬学科, 教授 (90272963)
• Co-Investigator(Kenkyū-buntansha): 佐藤 守 千葉大学, 医学部附属病院, 特任准教授 (20401002)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 臨床検査 / 質量分析 / ハイスループット / 誘導体化 / アナログ / アイソトポログ / 多検体一括 / バイオマーカー

Outline of Final Research Achievements

Mass spectrometry (MS) is accurate and economically-efficient methodology for clinical examinations, but its throughput is poor. This disadvantage deters the introduction of MS to the clinical practice. In this study, novel sample-multiplexed methods using sets of derivatization regent analogues and/or isotopologues were designed and developed to enhance the throughput of the MS-based clinical examinations for several diseases, such as vitamin D deficiency, estrogen-dependent cancer and primary aldosteronism. The developed methods could significantly reduce the analysis time (up to 60% less, compared with the conventional assays); thus the methods had a satisfactory applicability.

Free Research Field

分析科学

Academic Significance and Societal Importance of the Research Achievements

本研究により，MS臨床検査の最大の弱点であるスループットの低さが，適切にデザインされた誘導体化を基盤とする多検体一括定量，すなわち化学的アプローチによって克服できることが証明された．本成果は，各病院・検査機関が現有する装置を用いてハイスループットMS臨床検査が可能であることを意味しており，同時に検査手法がイムノアッセイから，正診度・医療経済性に優れるMSへと移行する流れを加速させるものと考えられる．