HIV-1 transcriptome analysis

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K09016
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kumamoto University
• Principal Investigator: SATO YORIFUMI 熊本大学, ヒトレトロウイルス学共同研究センター, 教授 (70402807)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: HIV-1

Outline of Final Research Achievements

First, we have established DNA-probe based viral sequence enrichment protocol for HIV-1 transcriptomic analysis by using cell lines. The efficiency was very high. We could enrich viral transcripts with more than 1,000 times.
Then, we applied the new protocol for clinical sample. We analyzed PBMCs from HIV-1-infected individuals. We analyzed them before and after cART. We found that even before cART, we could not detect enough number of HIV-1 transcripts even after enrichment.
We concluded that even our current is not good enough to detect HIV-1 RNA from clinical samples, especially PBMCs. We need to increase sensitivity or analyze different samples such as lymph nodes. We are going to continue this research h to make further progress.

Free Research Field

感染症内科学

Academic Significance and Societal Importance of the Research Achievements

HIV-1感染者において、抗ウイルス療法下で残存するウイルスリザーバの研究のために高感度ウイルス遺伝子発現検出系の開発を行った。従来の方法に比べ検出感度を数千倍高める事に成功した。
しかしながら、臨床検体でウイルスRNAを検出するには、それでも感度が不十分であったため、今後も研究を継続して、解析系の更なる改良を行う。