Analysis of exosomes released by exposure to metal oxide nanoparticles and their effects

2020 Fiscal Year Final Research Report

• Project/Area Number: 17K09165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Yogosawa Shingo 東京慈恵会医科大学, 医学部, 講師 (70381936)
• Co-Investigator(Kenkyū-buntansha): 柳澤 裕之 東京慈恵会医科大学, 医学部, 教授 (10200536)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: ナノ粒子 / 酸化亜鉛 / 皮膚 / 分化

Outline of Final Research Achievements

Metal oxide nanoparticles are widely used in cosmetics, but their exposure effects are also a concern. When zinc oxide nanoparticles were exposed to human skin keratinocyte HaCaT, the exosomes released by the cells were analyzed, and it was found that the particle size was smaller than usual, and that some keratins were included, suggesting that the cells were differentiated. We confirmed that the differentiation is PI3K/Akt pathway dependent with increase of involucrin. We also elucidated that zinc oxide nanoparticles induce cellular senescence. In addition, we found that the function of exosomes may differ among cells in their effect on cell migration.

Free Research Field

衛生学

Academic Significance and Societal Importance of the Research Achievements

酸化亜鉛ナノ粒子に曝露された細胞が通常とは異なるエクソソームを放出していることが判明した。またエクソソームの解析を通じて、酸化亜鉛ナノ粒子に曝露された細胞が分化することも判明した。細胞の局所的な分化誘導は皮膚の恒常性、バリア機能に影響する可能性もあるが、塗布する場合は、既に分化の進行した最外層の細胞に浸透するだけなので悪影響は少ないと考えられ、むしろその中で浸透した細胞におけるインボルクリン産生増加により、皮膚バリア機能が向上する可能性も考えられる。