Construction of basic data in toxicological evaluation: single and drug combinations used whole blood

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K09278
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Tokai University
• Principal Investigator: SAITO Takeshi 東海大学, 医学部, 准教授 (30266465)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 全血 / 血清 / 急性薬物中毒 / フェノチアジン / 珪藻土カラム

Outline of Final Research Achievements

We have compiled serum and whole blood concentrations for each compound found in acute toxicological cases. The results were characterized as follows: (1) Concentrations of many compounds were less than two times higher in serum than in whole blood, and the ratio remained constant regardless of concentration. (2) The ratio was 1 regardless of concentration. (3) The serum and whole blood concentration ratio was reversed with a decrease in concentration. Although, phenothiazine in the whole blood was not detected in extracted samples using K-solute cartridges (diatomaceous earth-based solid phase), methyl t-butyl ether was effective for whole blood extraction using K-solute. These results suggest that phenothiazines antipsychotics extraction from serum and whole blood using diatomaceous earth-based solid-phase columns seems to have an affinity with the elution solvent.

Free Research Field

法中毒

Academic Significance and Societal Importance of the Research Achievements

法医解剖に於いて急性薬物中毒の診断は血中薬物濃度によって評価されるが得られる試料は全血である。このため全血と血清の薬物濃度が比較できるデータは法医実務に応用可能である。また、死亡症例の多いフェノチアジン化合物の抽出過程によって化合物が酸化されるデータは実務上極めて重要でこれまで報告がみあたらない。これらのデータは、正しく診断が行われるために極めて有意義であり社会性も高い。