2019 Fiscal Year Final Research Report
Development of the forensic methylation panel to estimate the physical stress, age and body fluid type
Project/Area Number |
17K09281
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Legal medicine
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Research Institution | Kansai Medical University |
Principal Investigator |
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Keywords | メチル化解析 / 年齢推定 / 心臓突然死 |
Outline of Final Research Achievements |
The association of the ATP10A gene was suggested by genome-wide methylation analysis in the case of sudden infant death. Furthermore, methylation analysis of 16 CpG sites of 8 genes (ELOVL2, CCDC10, C1orf1, FHL2, ASPA, F5, KLF14, PDE4C) could estimate the age of the donor within ± 6 years. Finally, the "Methylation Panel" which could analyze these at once was established.
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Free Research Field |
法医遺伝学
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Academic Significance and Societal Importance of the Research Achievements |
死因の判断が困難な乳幼児の突然死例において,メチル化解析により新たなバイオマーカーとなり得るATP10A遺伝子の関連が示唆され,今後の研究が期待される.また,現場に残された生体試料から由来者の年齢推定が±6歳の精度で可能となり,犯罪捜査において非常に有用な方法が確立された.このように本研究の成果は,即座に法医実務に応用可能なものであり,メチル化解析の応用は今後さらなる発展が期待される.
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