2017 Fiscal Year Research-status Report

Aging deteriorates the function of lung stem cells, but it can be restored by calorie restriction.

Research Project

Project/Area Number	17K09666
Research Institution	Keio University
Principal Investigator	ヒガブ中井アハマド慶應義塾大学, 医学部(信濃町), 特任講師 (00507915)
Project Period (FY)	2017-04-01 – 2020-03-31
Keywords	lung stem cells / rachea stem cells / calorie restriction / aging
Outline of Annual Research Achievements	We have identified the effect of aging on lung tissue stem cells. We found that aging reduced number of basal stem cells in the trachea. We have achieved a successful calorie restriction protocol that caused both young and old mice to lose weight. We found that calorie restriction increased the number of basal stem cells in the trachea both in young and old mice. We successfully created a 3D cell culture technique that could mimic the in vivo calorie restriction.
Current Status of Research Progress	Current Status of Research Progress 1: Research has progressed more than it was originally planned. Reason The calorie restriction protocol went successfully so we did not need to repeat it several times to adjust. Samples collected showed clear statistical significance.
Strategy for Future Research Activity	We will examine the roles played by Sirt and mTOR genes, that are known to be related to calorie management. We will verify their roles in animal samples and in 3D culture system.
Causes of Carryover	ほとんど残っていません。