2019 Fiscal Year Annual Research Report
Aging deteriorates the function of lung stem cells, but it can be restored by calorie restriction.
| Project/Area Number |
17K09666
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| Research Institution | Keio University |
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Principal Investigator |
ヒガブ中井 アハマド 慶應義塾大学, 医学部(信濃町), 特任講師 (00507915)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | calorie restriction / lung stem cells / aging / colony-forming assay / mitochondria |
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| Outline of Annual Research Achievements |
we examined the effects of ageing on lung epithelial and stem cells and the effect of calorie restriction on young and old lungs. We found that ageing results in a decrease in tracheal basal stem cells. Calorie restriction induced an increase in basal stem cells in both young and old mice. In addition, ageing induced lung inflammation, and CR tended to reduce baseline lung inflammatory cell infiltration in young mice and significantly reduced ageing-induced lung inflammation. Furthermore, ageing reduced the number and function of mitochondria in lung and increased the level of mitochondrial reactive oxygen species. Calorie restriction increased the number and function of mitochondria both in young and old mice. Moreover, ageing reduced lung stem cell colony-forming efficiency (CFE), and calorie restriction increased the CFE in both young and old mice. Finally, calorie restriction improved epithelial cell survival in injured lungs of young mice. In conclusion, ageing causes several structural and functional changes/impairments in lung epithelial cells. calorie restriction induces several potentially beneficial changes in lung epithelial cells, even when it is initiated at an older age, including reversal of some ageing-induced changes.
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