Live imaging and molecular mechanism analysis of mouse embryonic brain development

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K10176
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Nagoya University
• Principal Investigator: saito kanako (齋藤加奈子) 名古屋大学, 医学系研究科, 特任助教 (50746906)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 大脳発生 / ライブ観察 / ニューロン移動

Outline of Final Research Achievements

This study, we elucidated that how the embryonic pallium expands ventrally to form the future cortex and the nature of the underlying force-generating events. We find that neurons born at embryonic day 10 (E10) in the mouse dorsal pallium ventrally stream, thereby superficially spreading the preplate, and then constitute the subplate. The preplate neurons migrate, exerting pulling and pushing forces at the process and the soma, respectively. Ablation of these E10-born neurons attenuates both deflection of radial glial fibers and extension of the cortical plate, which should occur ventrally, and subsequently shrinks the postnatal neocortical map dorsally. Thus, the preplate stream physically primes neocortical expansion and arealization.

Free Research Field

神経発生

Academic Significance and Societal Importance of the Research Achievements

本研究は、ヒトの先天性脳疾患の病態解明、および将来的な臨床診断ないし胎児治療の基盤となる知見を得るため、新規なライブ観察・解析の方法を確立するとともに、マウス大脳発生過程において研究代表者らが新たに見いだしたニューロン動態(接線方向移動)をリアルタイムに捉え、ニューロン移動の分子機構や意義を解明することで、胎児医学・先天異常学に新しい方法論と視点を提供できると期待される。