2021 Fiscal Year Final Research Report

3D analysis of epidermal barrier for improvement of skin barrier

Research Project

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Project/Area Number	17K10203
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Dermatology
Research Institution	Asahikawa Medical College
Principal Investigator	Ishida-Yamamoto Akemi 旭川医科大学, 医学部, 教授 (30241441)
Co-Investigator(Kenkyū-buntansha)	村上正基愛媛大学, 医学系研究科, 准教授 (20278302) 岸部麻里旭川医科大学, 医学部, 准教授 (90431410) 齋藤奈央旭川医科大学, 医学部, 研究生 (90736670)
Project Period (FY)	2017-04-01 – 2022-03-31
Keywords	keratinocytes / electron microscopy / lamellar granules / 3次元再構築 / focused ion beam / 連続切片走査電子顕微鏡法
Outline of Final Research Achievements	We have previously examined the distribution of epidermal lamellar granules (LG) by three-dimensional reconstruction of ultrastructural images using focused ion beam scanning electron microscopy (FIB-SEM), and found that LG-fusion with the plasma membrane was observed unevenly from the granular cell surface. In the present study, we examined the LG secretion system by serial section surface SEM, which visualized more expansive areas of the epidermis than FIB-SEM, and found that LGs were secreted first from the surface area of the cells under the more matured cells. This finding suggests that the granular cells can sense the maturation status of the cells facing above and start LG-secretion to the area facing the more matured granular cells.
Free Research Field	皮膚科学
Academic Significance and Societal Importance of the Research Achievements	超微細構造画像の3次元（3D）再構成の新しい技術には、集束イオンビームによるセクショニングアプローチを使用した集束イオンビーム走査型電子顕微鏡（FIB-SEM）や連続切片表面SEMがあるが、医学・生物学領域では超微細構造の3D再構築をもちいた研究成果の発表はまだ少ない。本研究では連続切片表面SEMを用いることにより、FIB-SEMよりも広い視野で細胞間の位置関係を繰り返し確認しながら、微細構造レベルで起きている細胞の変化を観察できることが示された。この方法は、他の細胞や組織でも応用できる手法であることから、この研究成果の学術的意義は高いと考えている。