Demonstration of transplanted Bone marrow derived mesenchymal stem/progenitor cells (MSCs) homing and co-effect of kinetic therapy on the spinal cord injury

2021 Fiscal Year Final Research Report

• Project/Area Number: 17K10943
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Showa University
• Principal Investigator: Hiraizumi Yutaka 昭和大学, 医学部, 客員教授 (10255870)
• Co-Investigator(Kenkyū-buntansha): 大滝 博和 昭和大学, 医学部, 准教授 (20349062) ; 渡邊 潤 昭和大学, 大学共同利用機関等の部局等, 兼任講師 (50649069) ; 吉川 輝 昭和大学, 保健医療学部, 講師 (90737355)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Keywords: 整形外科学 / 脊髄損傷 / 細胞移植 / 骨髄間葉系幹細胞 / リハビリテーション

Outline of Final Research Achievements

The patients subjected to spinal cord injury (SCI) are relatively younger age. Therefore, while the patients suffer for a long-periods, they have a potential to recover their lost functions by a regeneration. Bone marrow derived mesenchymal stem/progenitor cells (MSCs) have tried the clinical studies for the SCI. Moreover, the MSCs are known to modulate macrophage/microglial cells (MP/MG) polarization. However, it is still unclear about a communication between the MSCs and MP/MG, and the contribution of the chemokines. In present study, we determined hMSCs improved clinical symptoms after SCI and increased chemokines communicated with host parenchymal cells and the CCL5 which increased 14 says after SCI and hMSCs implant may contribute to MP/MG polarization for tissue repairing and axonal extension.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

脊髄損傷は若年齢患者が多い疾患である。それ故，患者が長期の不自由な生活になる一方，再生も期待される。骨髄間葉系幹・前駆細胞（MSCs）は脊髄損傷の臨床試験が行われている。一方でどのように機能回復が起きるのかその分子機構は不明である。報告者らはマウス脊髄損傷モデルを用いヒトMSCsを移植した際にMSCsは漸次的に低下するが生体内のマクロファージ・マイクログリアが細胞の遊走に関与するケモカインのCCL5の誘導を介して神経再生を促進する可能性を示した。