Analysis of aberrant microRNA expression due to the type of fusion gene in Ewing's sarcoma.

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K10971
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Oita University
• Principal Investigator: Kawano Masanori 大分大学, 医学部, 助教 (30571773)
• Co-Investigator(Kenkyū-buntansha): 田仲 和宏 大分大学, 医学部, 准教授 (10274458)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: Ewing肉腫

Outline of Final Research Achievements

Ewing sarcoma, a typical primary bone tumor, commonly develops in children and young adults, is highly malignant and has a poor prognosis, so we are awaiting development of new treatment methods. In Ewing sarcoma, the central component of the carcinogenesis mechanism is EWS-Fli1 working as an abnormal transcription factor, which requires a completely different treatment approach to conventional cancers. We will extract factors that increase or decrease across different subtypes, while simultaneously identifying factors where the expression changes do not match between subtypes, using the miRNA and mRNA extracted after EWS-Fli1 knock down. Through this research we plan to not only elucidate the carcinogenic mechanism of Ewing sarcoma, but also identify prognostic factors by EWS-Fli1 type, which we hope will lead to discovery of biomarkers and development of individualized treatment.

Free Research Field

骨軟部腫瘍

Academic Significance and Societal Importance of the Research Achievements

本研究により、type別にその下流遺伝子発現がどのように変化するのかを突き止めることが可能となる。EWS-Fli1に制御されEwing肉腫のがん化に寄与する因子、type 2の予後を悪化させる特異的な因子を同定、それらの生物学的意義を検証し、臨床応用への道を拓くことが期待できる。