Role of surfactant proteins A and D in host defense against uropathogenic Escherichia coli.

2020 Fiscal Year Final Research Report

• Project/Area Number: 17K11185
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Sapporo Medical University
• Principal Investigator: Ariki Shigeru 札幌医科大学, 医療人育成センター, 准教授 (80464478)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: 自然免疫 / コレクチン

Outline of Final Research Achievements

In this study, we analyzed the correlation between structure and function of lung collectins in host defense against uropathogenic Escherichia coli. The function of collectins dramatically changed depending on the composition of the analysis buffer. We also identified the region of the proteins required to exhibit the bacterial cell aggregation and the bacterial cell growth inhibitory activities. When the two types of lung collectins (SP-A and SP-D) coexisted, no synergistic effect was observed, but rather competitive effect was obtained.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、異所性に発現している肺コレクチンの機能を考察するうえで重要な知見になると考えられる。これらの知見を基にさらなる研究を行い、コレクチンによる尿路病原性大腸菌に対する自然免疫機構の全体像が明らかにできれば、尿路感染症のリスク評価や予防法の開発などにつながると期待される。