Analysis of reconstruction mechanism of endometrial epithelial cell sheet during human implantation

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K11251
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: HIHARA Hanako 慶應義塾大学, 医学部(信濃町), 助教 (80626458)
• Co-Investigator(Kenkyū-buntansha): 丸山 哲夫 慶應義塾大学, 医学部(信濃町), 准教授 (10209702) ; 内田 浩 慶應義塾大学, 医学部(信濃町), 講師 (90286534) ; 升田 博隆 慶應義塾大学, 医学部(信濃町), 講師(非常勤) (80317198) ; 内田 明花 慶應義塾大学, 医学部(信濃町), 助教 (60445236)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 着床

Outline of Final Research Achievements

During human implantation, embryo have to adhere and migrate into maternal endometrial tissue. For successful pregnancy, construction of embryo-penetrating route in endometrial epithelial cell barrier is required. In in vitro implantation assay system, the unique and reasonable preparation of embryo-penetrating route is performed by massive efferent motion of endometrial epithelial cells in association with endometrial epithelial cell apoptosis. The characteristic efferent cell motion is regulated by ovarian steroid hormones and can be accelerated by a kind of histone deacetylase inhibitors. Moreover, the acceleration ratio is higher in cells near the embryo attachment point compared to cells far from the central point.

Free Research Field

生殖医学

Academic Significance and Societal Importance of the Research Achievements

女性ホルモン剤投与による生殖補助医療の成功率上昇のメカニズムの一端を示している可能性がある。また、妊娠反応は認めても臨床的妊娠にはいたらないごくごく初期の流産（化学的流産）は、胚接着後間もない胚ー子宮内膜の相互反応の破綻ととらえることができるが、胚接着から胚陥入までの超初期段階の妊娠メカニズムとして子宮内膜上皮細胞の特異な細胞動態の解明は、今後の異常状態の理由（メカニズムのいかなる破綻か）の解明検討をあわせて臨床妊娠率向上にむけての新たな治療戦略の理論的基盤に発展していくことが期待される。