Investigation for the peritoneal factor in development of peritoneal endometriosis

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K11274
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kyoto University
• Principal Investigator: Tani Hirohiko 京都大学, 医学研究科, 助教 (70615252)
• Co-Investigator(Kenkyū-buntansha): 佐藤 幸保 京都大学, 医学研究科, 非常勤講師 (00508236) ; 伊藤 美幸 京都大学, 医学研究科, 特定病院助教 (00760951) ; 堀江 昭史 京都大学, 医学研究科, 講師 (30535836)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 腹膜因子 / 子宮内膜症 / in vivo / エストロゲン非依存性 / 持続分泌 / 生着期

Outline of Final Research Achievements

To investigate possible roles of peritoneal versican in the development of endometriosis using mouse model.Mouse model of developing endometriosis was prepared by intraperitoneally administration of endometrial tissue collected from donor mice to C57BL/6 8- to 9-week-old recipient mice. V1-CM or control-CM was intraperitoneallyinjected to model mice for continuous 5 days. Intraperitoneal endometriotic lesions were macroscopically confirmed. Experiments were approved by the Committee of the Institute for Animal Experimentation of Kyoto University Hospital. Results: Endometriotic lesions were observed in the peritoneal cavity of all mice. The total volume of the endometriotic lesion was significantly larger in V1-CM group than in the control group Conclusions: Our results suggest that peritoneal versican is involved in the developmentof peritoneal endometriosis.

Free Research Field

産科婦人科

Academic Significance and Societal Importance of the Research Achievements

本研究期間において腹膜因子であるversicanの子宮内膜症形成への関与をin vivoでの検証結果が得られ、versicanの発現がエストロゲン非依存性であることが示された。これらにより腹膜因子が子宮内膜症形成に関与することがより強く示唆され、一連の検証モデルが他の腹膜因子検証に用いることができる確証が得られた。