2019 Fiscal Year Final Research Report

Antioxidative effect of ascorbate peroxidase derived from Cyanidioschyzon merolae in mammalian cells

Research Project

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Project/Area Number	17K11648
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Functional basic dentistry
Research Institution	Kyushu Dental College
Principal Investigator	Hitomi Suzuro 九州歯科大学, 歯学部, 講師 (70548924)
Co-Investigator(Kenkyū-buntansha)	小野堅太郎九州歯科大学, 歯学部, 教授 (40316154) 古株彰一郎九州歯科大学, 歯学部, 教授 (30448899) 松本謙一郎国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所放射線障害治療研究部, グループリーダー(定常) (10297046)
Project Period (FY)	2017-04-01 – 2020-03-31
Keywords	ascorbate peroxidase / Cyanidioschyzon merolae / oxidative stress / reactive oxygen species
Outline of Final Research Achievements	Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, which is a primitive red alga living in high-temperature and acidic environment, has been reported greater anti-oxidative capacity than those in other plants. In the present study, we examined a possibility to increase anti-oxidative capacity in mammalian cells by the expression of Cyanidioschyzon merolae-drived APX (cAPX). cAPX gene was introduced into the mice fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability after heat, H2O2 and acid stimulations were assessed. Heat and H2O2 stimulations caused ROS production. cAPX-expressed cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells. From these results, introduction of cAPX increases antioxidative capacity in mammalian cells.
Free Research Field	口腔生理学
Academic Significance and Societal Importance of the Research Achievements	本研究結果は、動物細胞におけるAPXの機能解明に役立つだけではなく、がん治療を原因として局所に産生される過剰な活性酸素種（ROS）の除去に応用できる可能性がある。がんの放射線治療や化学療法では、がん細胞に対する ROSの傷害作用を抑制することなく、がん細胞以外の“局所”でROSを調節することが望ましい。今後、cAPX発現細胞を用いた細胞シートなどによる粘膜保護が可能になれば、粘膜細胞が酸化ストレスに強い抵抗性を獲得し、酸化ストレスを原因とした粘膜潰瘍や疼痛の抑制に役立つ可能性がある。