2020 Fiscal Year Final Research Report

Elucidation of the mechanism of irradiated tooth germ formation disorder

Research Project

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Project/Area Number	17K11687
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Pathobiological dentistry/Dental radiology
Research Institution	The Nippon Dental University
Principal Investigator	Iwata Hiroshi 日本歯科大学, 生命歯学部, 准教授 (40328860)
Co-Investigator(Kenkyū-buntansha)	井出吉昭日本歯科大学, 生命歯学部, 准教授 (70409225) 深田哲也日本歯科大学, 生命歯学部, 助教 (20386254) 中原貴日本歯科大学, 生命歯学部, 教授 (10366768)
Project Period (FY)	2017-04-01 – 2021-03-31
Keywords	放射線被爆 / 歯胚
Outline of Final Research Achievements	In order to elucidate the mechanism of tooth germ formation disorder due to irradiation exposure, we examined an irradiation experimental model on embryonic mice. We investigated a method of locally irradiating the abdomen of pregnant mice (in vivo), a method of temporarily removing the fetus from the uterus of the pregnant mouse (in vivo) and irradiating it back to the mother, and an organ culture method (in vitro).In vivo experiments, there were many variations in control mice due to radiation damage in pregnant and fetal mice and the effects of experimental techniques. Irradiation experiments using the organ culture method were considered to be suitable for this study.
Free Research Field	歯科放射線
Academic Significance and Societal Importance of the Research Achievements	小児期の放射線被曝は矮小歯、エナメル質減形成、歯胚の無形成（石灰化不全）などが生じると報告されている。今までに実験動物を扱い、放射線が歯の発生に与える影響について報告されているが、歯の形成障害のメカニズムを解明したものではない。本研究では、放射線被曝による歯冠形成障害のメカニズムを明らかにすることを目指し、その実験モデルの開発を行う。本研究を行うことにより、放射線被曝による歯胚形成障害の予防法や治療法の開発に寄与できると考える。