Fundamental studies on the ability of Lactobacillus species to synthesise corinoid compounds.

2021 Fiscal Year Final Research Report

• Project/Area Number: 17K12889
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: TANIOKA YURI 東京農業大学, 国際食料情報学部, 准教授 (30553250)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Keywords: コリノイド合成乳酸菌 / 人工消化

Outline of Final Research Achievements

Corrinoid-producing Lactobacillus in this study didn’t produce true B12 but pseudovitamin B12 inactive for human. When cobalt and glutamine, which are necessary for corrinoid biosynthesis, were added to the medium and the corrinoid compounds produced were examined, an increase in corrinoid production and growth enhancement were observed. The effect of corrinoid-producing Lactobacillus on the tolerance of artificial digestion found to be reduced to 20% at pH 2, and the addition of cobalt or glutamine to the medium did not change the survival rate.

Free Research Field

食品科学

Academic Significance and Societal Importance of the Research Achievements

ヒト糞中には、B12以外の下方配位子の塩基が異なる多様なコリノイドが存在することが報告されており、腸内細菌はB12やそれ以外のコリノイドを利用、またはリモデリングしていると考えられる。本研究で得られたコリノイド生産乳酸菌の人工消化耐性やコリノイド化合物の基礎的な情報は、新規プロバイオティクス素材としての可能性を示した成果になると考えられる。