Effect of extracellular vesicles in milk on colon cancer cell proliferation

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K12899
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Okayama University
• Principal Investigator: Tsuruta Takeshi 岡山大学, 環境生命科学研究科, 准教授 (90728411)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 牛乳 / 細胞外小胞 / 大腸がん

Outline of Final Research Achievements

Several prospective cohort studies suggested that milk might possibly have a preventive effect on colorectal cancer. In this study, the effect of extracellular vesicles (EVs) in bovine milk on growth activity of colon cancer cell line HT29 and tumorigenesis in colorectal carcinogenesis model were evaluated. As a result, milk EVs suppressed the growth activity of HT29 in a dose dependent manner possibly via cell cycle arrest at G1 phase. Furthermore, oral administration of milk EVs to colorectal carcinogenesis model mice tended to decrease the number of colorectal tumor.

Free Research Field

食品機能学

Academic Significance and Societal Importance of the Research Achievements

本研究では、牛乳による大腸がんの発症予防効果に科学的根拠をともなわせるために牛乳中のEVsに着目し、研究を進めてきた。培養細胞や実験動物を用いた検証結果から、牛乳EVsが大腸がんの発症予防効果の一端を担っている可能性が示された。本研究結果は消費者の生活に広く浸透している食品である牛乳に対して大腸がん予防という新たな可能性を提示するものである。