2019 Fiscal Year Final Research Report
Elucidation the effect of biotin on palate development through an epigenetic mechanism and the prevention of cleft palate
Project/Area Number |
17K12906
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Eating habits
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Research Institution | University of Hyogo |
Principal Investigator |
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Keywords | ビオチン / 口蓋裂 |
Outline of Final Research Achievements |
Cleft palate is one of the most common congenital craniofacial anomalies in Japanese which is caused by genetic and environmental factors. However, the exact cause and mechanism of cleft palate has not yet been elucidated. Pregnant mice were given a biotin-deficient diet and then were changed to a biotin-supplemented diet on day 10, 11 or 12 of gestation. The incidence of cleft palate was lower in the dg 10-supplemented group and the dg 11-supplemented group than the biotin-deficient group. It is suggested that biotin is necessary to develop and fuse the palatal process in mouse fetuses at midgestation (dg 11 and earlier). We speculated that biotin may play a role in the initial stage of palatal development.
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Free Research Field |
分子栄養学
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Academic Significance and Societal Importance of the Research Achievements |
口蓋裂の研究は、疾患遺伝子の同定によって遺伝要因に関する研究は進んでいる反面、環境因子による詳細なメカニズムの報告が少ないのが現状である。本研究では、口蓋裂発生とヒストンのビオチン化およびメチル化との関連に着目し、新たな口蓋裂発生メカニズムを探索することで、環境因子による口蓋裂発生のメカニズムに関する新たな知見の提供を目指す。また、本研究において口蓋形成におけるビオチンの役割を解明することは、妊娠期におけるビオチン摂取の重要性の提唱につながり、将来的には妊娠期のビオチン補充が口蓋裂発生予防につながる可能性が期待できる。
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