2018 Fiscal Year Annual Research Report
High efficient myogenic differentiation of human pluripotent stem cells on New Generation Laminins towards regenerative medicine
Project/Area Number |
17K13014
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Research Institution | Kyoto University |
Principal Investigator |
Zhao Mingming 京都大学, iPS細胞研究所, 特定研究員 (50754206)
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Project Period (FY) |
2017-04-01 – 2019-03-31
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Keywords | 再生医学 / 次世代ラミニン / iPS細胞 / 骨格筋幹細胞 |
Outline of Annual Research Achievements |
We established a system for differentiating hiPSCs on the new generation laminin (NGL). In this system, HS bind to fibroblast growth factor-2 (FGF2), form a stable high affinity HS-FGF2-FGFR complexes on cell surface, then strongly stimulate FGFR signaling pathway. NGL time-dependently increases the marker genes expression of primitive streak and paraxial mesoderm lineage. These effects depend on the unique structure of NGL, that could not be replaced by treating with high dose of FGF2 or coating the mixture of laminin421 E8 and perlecan. Using this xeno-free matrix, we established a highly efficient differentiation system for hiPSCs induced paraxial mesoderm lineage, subsequently, paraxial mesoderm derived myocytes and muscle stem cells.
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Research Products
(4 results)