Development of a sensitive and easy method for analysis of organic multivalent ions using an enzyme

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K14503
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Fukui Prefectural University
• Principal Investigator: Uematsu Kohei 福井県立大学, 生物資源学部, 講師 (30547584)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: 多価カチオン / ポリアニオン / 酵素 / 比色分析

Outline of Final Research Achievements

Useful natural and synthetic multivalent ions widely exist, and an easy and high sensitive analytical method for the multivalent ions has been required. The representative researcher previously found a significant promotion effect of ε-poly-L-lysine on an enzymatic reaction, and reveal that the effect is due to the polycationic property of ε-poly-L-lysine. In this study, based on the information, a novel analytical method for multivalent ion using enzyme has been developed. The method allowed for the determination of a multivalent cation at 0.1 ppm level, and was also capable of determining polyanions at 1μeqM level.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

酵素を用いて多価イオン物質を簡便かつ高感度に分析できる手法を開発した。同法は市販の試薬を混ぜるだけで可能であり，多価カチオンの場合では，吸光度計を用いれば0.1 ppmレベルでの定量分析が行える。目視でも同レベルの半定量分析が行え，他の多価イオン比色分析法と比較して十分高い感度を示す。本法はその操作の簡便さと高い感度特性から，多価イオンが関与する基礎・応用研究ならびに品質管理などの実際的利用において，極めて有効な分析法の一つになりうると期待される。