2017 Fiscal Year Research-status Report
多能性幹細胞由来心筋永久機関：循環進行波に基づく加速心筋細胞成熟法の開発
| Project/Area Number |
17K14624
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| Research Institution | Osaka University |
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Principal Investigator |
李 俊君 大阪大学, 医学系研究科, 特任研究員(常勤) (10723786)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | pluripotent stem cells / cardiomyocytes / maturation |
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| Outline of Annual Research Achievements |
In the last year, our projects progressed smoothly. We have design and optimized the self-organized tissue ring technology for promoting high-speed cardiomyocytes beating. This technique could be used for rapid and controllable cardiac maturation without any external stimulation.The achievements are specifically:
1.Different parameters have been discussed for controlling the beating frequency of cardiomyocytes. Up to 90% percent success rate could be achieved in the multi-well plate. 2.The Traveling wave could work in several frequency mode and beat for more than 3 months. 3.A computational model has been created by using Matlab. The model could interpret the mechanism how the traveling waves are generated and maintained.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
So far, we have almost achieved all the planned step for first year. We are optimistic that the project could progress smoothly in the following year.
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| Strategy for Future Research Activity |
Based on previous work, we will further characterize the maturation and performance of cardiomyocytes in self-organized tissue ring after training. The Real-time PCR, immunostaining, transmission electron microscope,contractile force generation and other methods will be used to characterize the maturation of cardiomyocytes. The data will be compared with the other reports.
2. Large-scale production method for matured tissue ring will also be established. Rings made of ~10e7 cardiomyocytes will be made within one plate.The design of plating well will be optimized to improve the robustness of traveling wave. The interruption of accidental conditions will be minimized by improving the well structure.
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