Circulating traveling wave enhances the maturation of cardiomyocytes in self-organized tissue

2018 Fiscal Year Annual Research Report

• Project/Area Number: 17K14624
• Research Institution: Osaka University
• Principal Investigator: 李 俊君 大阪大学, 医学系研究科, 特任研究員(常勤) (10723786)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: cardiomyocyte maturation / organ on chip / tissue engineering / iPS cell

Outline of Annual Research Achievements

The targets in proposed plan have been well fulfilled specified as following:1. A low-attachment substrates have been designed and optimized to create cardiac tissue ring with spontaneous traveling wave. 2. The cardiomyocytes trained by traveling wave demonstrated matured sarcomere structure, up-regulated gene expression, improved calcium handling properties and mitochondrial function. 3. A systems compatible to 24-well cell culture dish have been created for up scaled production of ring. 4. One Japanese patent has been submitted. “Circulating traveling waves enhance the maturation of hiPSC-derived cardiomyocytes in self-organized tissue ring” has been sent out for review in Science Advance. Two other papers supported by the grant have been also published.

Research Products

• [Int'l Joint Research] Perking University(中国)
  • Country Name: CHINA
  • Counterpart Institution: Perking University
• [Journal Article] Clonal Isolation of Human Pluripotent Stem Cells on Nanofibrous Substrates Reveals an Advanced Sub-clone for Cardiomyocyte Differentiation (2019)
  • Author(s): Yu, L. ; Li, J. ; , Minami, I.; Qu X.; Miyagawa S.; Fujimoto, N.; Hasegawa K.; Chen, Y.; Sawa, Y.; Kotera, H.; Liu, L.,
  • Journal Title: Advanced Healthcare Materials Volume: In press Pages: In press
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Low Cell-Matrix Adhesion Reveals Two Novel Subtypes of Human Pluripotent Stem Cells. (2018)
  • Author(s): Yu, L. ; Li, J.; Hong, J.; Takashima, Y.; Fujimoto, N.; Nakajima, M.; Dong, X.; Dang, Y.; Yang, W.; Okita, K.; Luo, C.; Tang, F.; Chen, Y.; Tang, C.; Kotera, H.; Liu, L.,
  • Journal Title: Stem Cell Reports Volume: 11 Pages: 142-156
  • DOI: https://doi.org/10.1016/j.stemcr.2018.06.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 組織工学技術による新規心筋成熟法の開発 (2019)
  • Author(s): 劉 莉
  • Organizer: 日本再生医療学会
• [Patent(Industrial Property Rights)] Ring-like cardiac tissue construct (2018)
  • Inventor(s): Liu, L.; Li, J.
  • Industrial Property Rights Holder: Liu, L.; Li, J.
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: Liu, L.; Li, J.