2018 Fiscal Year Annual Research Report
The Emergence and Control of Olfactory Circuits in Development
| Project/Area Number |
17K14946
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| Research Institution | Kyushu University |
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Principal Investigator |
LEIWE MARCUS 九州大学, 医学研究院, 助教 (80722008)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | Olfactory Bulb / Olfaction / Odour / Mice / Neurodevelopment |
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| Outline of Annual Research Achievements |
During the last two years, several acheivements have been made.
Firstly, we were able to uncover a key role for the NR1 subunit in mitral cell dendrite pruning. Not only does this retain the multiple dendrites but it also changes the pattern of spontaneous activity. Highlighting the key link between structure and function.
Secondly, we obtained preliminary data for olfactory development. To do this we built an odour delivery machine that was able to sync to our 2 photon microscope, then in conjunction with this we devised a method of recording the respiration rate of neonatal mice down to P2. We are able to analyse these three systems simultaneously and so can measure the responses in relation to actual inhilation time. Then by selecting three key odours we have begun to look at the key changes in the patterns of odour responses at different ages. We can see how maximal responses change as well as the underlying circuit activity. Further analysis is underway in order to asess the link to respiration, in other words the phase code.
Thirdly, using the triple mutant DAT-Cre-TeNT-Thy1 GCaMP6f mice we have analysed the effects of inhibiting dopaminergic activity on odour responses. Unfortunately as these mice die at P21 our investigations will have to be matched to this juvenile age.
Finally, we have been able to create a way of hand rearing pups to normal development. This allowed us to carry out further manipulations that would have resulted in the maternal rejection of the pups.
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