2018 Fiscal Year Annual Research Report
Establishment of new diagnostic method for HTLV-1 related diseases using MinION nanopore sequencer
| Project/Area Number |
17K15044
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Firouzi Sanaz 東京大学, 大学院新領域創成科学研究科, 客員共同研究員 (30793400)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | HTLV-1 / ATL / Personalized Medicine / Intratumor Heterogeneity / TCR clonality / Integration Site / Nanopore sequencing / Clonality analysis |
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| Outline of Annual Research Achievements |
We employed various multi-omics approaches to determine clonality structures of HTLV-1 infected individuals using three different criteria including the provirus integration sites, T cell receptor rearrangements and somatic mutations. ① Clonality analysis based on integration sites using NGS technology suggested its possible applications in molecular diagnosis,and predicting prognosis (Blood advances 2017,Human genomics 2017).We also developed a new and rapid method for reliable clonality analysis using MinION Nanopore sequencer.② We showed ATL displays a high degree of ITH and a complex subclonal structure based on mutation(Neoplasia 2018)③ We found that TCR clonality profiling by RNA sequencing is useful for prognostic purposes and personalizing ATL diagnosis (npj genom. Med. 2019).
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