2017 Fiscal Year Research-status Report
Novel immune organs producing innate lymphoid cells - function and abnormality of mediastinal fat-associated lymphoid clusters -
| Project/Area Number |
17K15388
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| Research Institution | Hokkaido University |
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Principal Investigator |
エレワ ヤセル 北海道大学, 獣医学研究院, 助教 (30782221)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | mediastinal fat / MFALCs / Bleomycin / C57BL/6 mice / MRL/MpJ-faslpr mice / Lung inflammation / Lung fibrosis / sex differences |
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| Outline of Annual Research Achievements |
Achievement during FY2017:
1-I revealed the sex-related differences in autoimmune-induced lung lesions in MRL/MpJ-faslpr mice and its relationship with the development of mediastinal fat-associated lymphoid clusters (MFALCs) (Elewa et al. 2017, Autoimmunity).
2-Interestingly, it has been previously revealed that the B6 mice showed susceptibility to development of lung fibrosis following bleomycin (BLM) administration and showed well developed MFALCs in normal condition (Elewa et al. 2014). However, the correlation between MFALC morphologies and lung lesion progression, remain to be clarified. Therefore, recently I revealed the correlations between MFALCS and the development of lung inflammation and fibrosis in BLM-induced pneumonitis B6 mice model (Elewa et al. 2018, Front. Immunol.).
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
1-Because of the financial provided, we could buy different mice strains (including Male and female MRL/MpJ-faslpr autoimmune mice model, and male B6 strains), different antibodies for performing immunohistochemistry for characterizing different immune cells (T, and B-lymphocytes, macrophages, granulocytes) and vessels (lymphatic vessels and high endothelial venules) and different chemicals (bleomycin, and PCR reagents).
2-Collaboration with other laboratory members for applying new techniques.
3-Availability of the place for maintaining the mice and applying the experiments.
4-Having chances to present the obtained data and results in both international and local conferences.
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| Strategy for Future Research Activity |
1-Based on our recent report of the role of MFALCs in the development of lung inflammation and fibrosis following bleomycin administration (Elewa et al. 2018). Furthermore, based on our previous observation of well-developed MFALCS in autoimmune mice model than its control strains and its relationship with the development of lung lesions (Elewa et al. 2016). However, the effect of bleomycin administration on the development of MFALCS and lung lesion is still unclear. Therefore, we plan to examine their effect on two autoimmune mice models (MRL/MpJ-faslpr and BXSB/MpJ‐Yaa mice) and compare with those of the corresponding control strains (MRL/MpJ and BXSB/MpJ, respectively).
2-We plan to examine such MFALCs and its relationship with lung allergy and asthma (papain induced asthma model).
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| Causes of Carryover |
The remaining amount of money in this fiscal was very small (137 yen) that is not enough to buy needed chemicals.Therefore, we kept it to be used with that of the next fiscal year.
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