2018 Fiscal Year Research-status Report
Novel immune organs producing innate lymphoid cells - function and abnormality of mediastinal fat-associated lymphoid clusters -
| Project/Area Number |
17K15388
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| Research Institution | Hokkaido University |
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Principal Investigator |
エレワ ヤセル 北海道大学, 獣医学研究院, 助教 (30782221)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | Bleomycin (BLM) / C57BL/6 mice / DBA/2mice / lung injury / lung inflammation / MFALCs / sublingual immunotheraby / beads |
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| Outline of Annual Research Achievements |
Achievement during FY2018:
1-I clarified the correlations between MFALCS and the development of lung inflammation and fibrosis in bleomycin (BLM)-induced pneumonitis B6 mice model (Elewa et al. 2018, Front. Immunol.).However,the effect of BLM on the degree of lung injury and development of MFALCs is not yet clarified. Therefore, in the current experiment I designed experiment to clarify its effect .
2. I revealed the Morphofunctional analysis of antigen uptake mechanisms following sublingual immunotherapy with beads in mice(Elewa et al. 2018, PLOS ONE).
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
1-Due to available grant fund, we could buy different mice strains (including Male and female MRL/MpJ-faslpr autoimmune mice model, and male B6 strains), different ELISA kits, different antibodies for performing immunohistochemistry for characterizing different immune cells (T, and B-lymphocytes, macrophages, granulocytes) and vessels (lymphatic vessels and high endothelial venules) and different chemicals (bleomycin, and PCR reagents).
2-Collaboration with other laboratory members for applying new techniques.
3-Providing different agreement to have collaboration with other laboratories as well as importing some samples.
4-Availability of the animal facilities for maintaining the mice and applying the experiments.
5-Presenting my data and results in both international and local conferences.
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| Strategy for Future Research Activity |
1-I designed experiment for examining of the effect of bleomycin administration on two autoimmune mice models (MRL/MpJ-faslprand BXSB/MpJ‐Yaa mice) and compare it with those of the corresponding control strains (MRL/MpJ and BXSB/MpJ, respectively). Therefore, currently I am planning to analyse the data and results degree of lung injury score among all groups as well as the immune cell infiltration in lung and its correlation with that of the MFALCs following BLM administration at 7 and 21 d.
2-Furthermore, planning to analyse the role of MFALCs in the susceptibility of C57BL/6 and DBA/2 mice to Mycoplasma pulmonis infection.
3-We plan to examine such MFALCs and its relationship with lung allergy and asthma (papain induced asthma model).
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| Causes of Carryover |
The remaining amount of money in this fiscal was very small (1144 yen) that is not enough to buy needed chemicals.Therefore, we kept it to be
used with that of the next fiscal year.
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[Journal Article] Histopathological Correlations between Mediastinal Fat-Associated Lymphoid Clusters and the2018
Author(s)
Elewa, Y. H. A., Ichii, O., Takada, K., Nakamura, T., Masum, Md.A., Kon, Y.
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Journal Title
Frontier Immunology
Volume: 9 (271)
Pages: 1-10
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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