2017 Fiscal Year Research-status Report
Identification of non-invasive novel biomarkers predicting future asthma exacerbations in adults and early-onset wheeze in children
| Project/Area Number |
17K15843
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | Asthma / Urinary biomarkers / birth cohort / adult asthma cohort |
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| Outline of Annual Research Achievements |
In our adult asthma cohort (HiCARAT), we examined hexanoyl-lysin (HEL), propanoyl-lysine (HNE) in urine samples of adult asthma patients. HEL and HNE are indicators of omega 6 and omega 3 fatty acid peroxidation, respectively. Urinary HEL and HNE levels were (median and IQR) as follows: 52.3 (38.3-85.9) nmol/L and 3.2 (0.7-12.5) μg/mL. Additionally, we measured urinary creatinine to adjusted HEL and HNE levels. Detection rate for HEL and HNE were 100% and 90%, respectively. Coefficient of variation was less than 10% for measurement which indicates high quality of biomarker measurements in our study. Urinary levels of these two biomarkers were highly correlated. We stratified patients by BMI (less or more than 25 kg/m2) and asthma severity (severe vs. non-severe) into four groups. We found monotonic trend for increasing levels of HEL and HNE across the following four groups: BMI less than 25 and non-severe; BMI more than 25 and non-severe asthma; BMI less than 25 and severe asthma; BMI more than 25 and severe asthma. These biomarkers we associated with higher BMI and asthma severity. We are currently conducting data analysis regarding association of these biomarkers with future risk of exacerbations.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
Our project is going very well.
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| Strategy for Future Research Activity |
Birth cohort: Using ISAAC for children, we have collected information of wheeze, rhinoconjunctivitis, and eczema at age 7. Using children's urine samples, we have also measured three biomarkers including 8-hydroxy-2 deoxyguanosine (8-OHdG), HNE and HEL. In our adult asthma cohort (HiCARAT) we measured urinary HNE and HEL and we found very promising data. Therefore, we applied these two biomarkers in urinary sample of children, as well.
Urinary levels (median and IQR) of 8-OHdG (ng/mL), HEL (nmol/L) and HNE (μg/mL) were 10.3 (7.5-13.2), 119.9 (77.2-179.4), and 52.8 (27.4-113.0). Detection rate for these three urinary biomarkers were 100%.
Currently, we are conducting data analysis to address association of childhood allergic conditions and BMI with these urinary biomarkers.
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| Causes of Carryover |
We are collaborating with several groups in adult asthma cohort and birth cohort. In this fiscal year, we are going to measure more biomakers and use this grat to cover the expenses.
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