Elucidation of stem cell pathogenesis in inflammatory bowel disease using primary intestinal epithelial stem cells culture and development of new therapeutic agent

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K15930
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: NISHIMURA Ryu 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60778132)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: 幹細胞機能評価 / 初代培養 / 炎症性腸疾患

Outline of Final Research Achievements

We have established in vitro UC model using human colonic organoid. Microarray analysis showed a significant induction of inflammatory-related genes in colonic organoids whereas cell differentiation was suppressed. Treatment with the investigational drug showed reciprocal dynamics of gene expression to inflammatory stimulation. We by focused a series of genes that were upregulated in an antagonistic response to inflammation by treatment with the investigational drug, and identified the molecular target.
Further maturation of this system might be more efficient to predict the effect on UC for the development of new drugs.

Free Research Field

消化器病態学

Academic Significance and Societal Importance of the Research Achievements

体外でのヒト大腸上皮細胞でも潰瘍性大腸炎モデルの作成に成功し、潰瘍性大腸炎における大腸上皮細胞の病態の一部が明らかになった。このモデルを用いて新規治療候補薬の効果や作用機序を明らかにすることができた。このモデルを用いて治療候補薬のスクリーニングや安全性の評価することが期待できるものと考えられる。