2018 Fiscal Year Final Research Report
Endoscopic and molecular analysis in colorectal serrated lesions
| Project/Area Number |
17K15959
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Research Collaborator |
SUZUKI Hiromu
YAMAMOTO Eiichiro
Aoki Hironori
YAMANO Hiro-o
SUGAI Tamotsu
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | 大腸がん / 前癌病変 |
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| Outline of Final Research Achievements |
We aimed to identify epigenetic alterations that are associated with the development of TSA. The Infinium HumanMethylation450 (HM450) BeadChip array analysis identified that SMOC1 gene was frequently methylated in TSAs. RT-PCR revealed that SMOC1 is abundantly expressed in normal colon and SSA/Ps, but is significantly downregulated in TSAs. Immunohistochemical analysis showed that SMOC1 was expressed in the epithelium of normal colonic tissues and SSA/Ps, but that expression is significantly reduced in TSAs.Methylation of SMOC1 is associated with TSA development but is rarely observed in SSA/Ps. Immunohistochemical analysis of SMOC1 may be a useful marker to discriminate between SSA/Ps and TSAs. Our data suggests SMOC1 methylation may play a role in the neoplastic pathways arising in TSAs.
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| Free Research Field |
エピジェネティクス
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| Academic Significance and Societal Importance of the Research Achievements |
SMOC1のメチル化、発現が鑑別困難な鋸歯状病変の診断バイオマーカーとして役立ち、SMOC1のメチル化が大腸がん発症リスクの高い前駆病変の予測マーカーになり得ることが示唆される。発がんリスクの高い前癌病変が同定することができれば発がん予防、大腸がん死減少に貢献できるものと考える。
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