Analysis of tumor-associated microRNAs in hyperparathyroidism

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K16099
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Tokai University
• Principal Investigator: KANAI Genta 東海大学, 医学部, 講師 (00535221)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 副甲状腺機能亢進症 / microRNA / 次世代シーケンサー / 慢性腎臓病

Outline of Final Research Achievements

In this study, to identify miRNAs that regulate function through parathyroid receptors, we examined whether miRNAs associated with tumorigenesis and functional regulation could serve as predictors of SHPT progression and markers of treatment resistance.
RNA extracted from the maximal and minimal glands of the parathyroid gland from a patient with secondary hyperparathyroidism was used for comprehensive analysis of miRNAs using NGS RNA sequencing.
In addition to miRNAs with known functions among 20 representative sequences, four new sequences showed significant differences in function. in vitro, these sequences were examined for function against target mRNAs with complementary sequences in the 3'UTR region, and an action associated with p21/27 was observed.

Free Research Field

腎臓内科学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果によって副甲状腺の段階的腫瘍化仮説における遺伝子発現調節機序の一端が解明されることで、難治性の二次性副甲状腺機能亢進症における進展メカニズムを理解する一助となり、患者の生命予後にかかわる慢性腎臓病における骨ミネラル代謝異常の治療戦略における新たな基礎的知見を与えるものであると考えられる。