2018 Fiscal Year Annual Research Report
The underlying mechanisms of synaptic dysfunction after the irradiation
| Project/Area Number |
17K16424
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| Research Institution | Gunma University |
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Principal Investigator |
P Anggraeini 群馬大学, 未来先端研究機構, 助教 (60782050)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | radiation / synaptic dysfunction / synaptic proteins / neurons / hippocampus |
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| Outline of Annual Research Achievements |
Previously we have shown that radiation may produce acute temporary cognitive impairment via decreases of postsynaptic protein drebrin from the synapse within 24 hours. To reveals the mechanisms radiation-induced synaptic dysfunction and its prevention, the mice were pretreated by injection of saline or N-methyl-D-aspartate (NMDA) receptor antagonist MK801 ten minutes before of a whole brain of ten-weeks-old C57BL/6N male mice, then we examine the synaptic function using drebrin, and PSD-95 immunoreactivity on DG of Hippocampus 8 hours following X irradiation. Our results show there was a decrease in the immunoreactivity of drebrin and prevented by MK801. Thus results indicate that indicates that NMDA receptor mediates X-irradiation-induced drebrin decrease possibly via drebrin exodus.
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Research Products
(11 results)
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[Presentation] Radiation effects on neurons and its consequences: investigation by in vitro, in vivo and in silico studies.2018
Author(s)
Anggraeini Puspitasari, Hiroyuki Yamazaki, Noriko Koganezawa, Nobuhiko Kojima, Hidemasa Kawamura, Aimee Louise McNamara, Jan P Schuemann, Harald Paganetti, Tomoaki Shirao, Takashi Nakano, Kathryn D Held.
Organizer
3rd FARO MEETING 2018, Bali, Indonesia
Int'l Joint Research
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