Fabrication of pancreatic islet-like tissue using layer-by-layer cell coating technique

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K16507
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: Fukuda Yasunari 大阪大学, 医学部附属病院, 医員 (20768973)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: 交互積層細胞コート法 / スフェロイド / 膵β細胞 / インスリン感受性 / コネキシン36

Outline of Final Research Achievements

Development of emerging techniques in tissue engineering for pancreatic β-cells alternatives to islet transplantation is becoming incresingly important. The purpose of this study is to fabricate functional islet-like tissue using layer-by-layer (LbL) cell coating technique in combination with sheroid technique using a mouse pancreatic β-cell line. The well-structured and dense spheroids could immediately be constructed by the LbL-coated β-cells. In the functional analysis, spheroids with the LbL-coated β-cells improved the insulin sensitivity compared to spheroids with non-coated β-cells. When spheroids with the LbL-coated β-cells were syngeneically transplanted in diabetic mice, glucose sensitivity improved after intraperitoneal glucose stimulation compared to spheroids with non-coated β-cells. This cell coating technique would be a frontier approach in fabricating clinically applicable islet-like tissue.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

大阪大学大学院　生命機能研究科　明石満　教授らにより開発された細胞外マトリックスによる交互積層細胞コート法は、手技が簡便で、生体適合性が高い細胞表面修飾技術である。この交互積層細胞コート法にスフェロイド技術を組み合わせることで、早期から細胞密度が高く機能性が高い膵島細胞類似組織の構築が可能であった。今後、ES細胞やiPS細胞由来の膵β細胞を細胞資源にして本手法を用いることで臨床応用可能な膵島細胞類似組織を構築し、重症I型糖尿病患者の治療に大いに役立つことが期待される。