2018 Fiscal Year Final Research Report
Establishment of primary culture of gastrointestinal tumor for clinical application
Project/Area Number |
17K16542
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Osaka University |
Principal Investigator |
Fujino Shiki 大阪大学, 医学系研究科, 特任助教(常勤) (10768956)
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Research Collaborator |
Miyoshi Norikatsu
Ito Aya
Saso Kazuhiro
Sasaki Masaru
Matsuura Nariaki
Ohue Masayuki
Yasui Masayoshi
Mori Masaki
Doki Yuichiro
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Project Period (FY) |
2017-04-01 – 2019-03-31
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Keywords | 初代培養 / 癌 / 大腸癌 / 個別化医療 / 薬剤感受性 |
Outline of Final Research Achievements |
Tumors and primary cultured cells show phenotypic heterogeneity, and two-dimensional (2D) cultured cells are useful for various assays used to study these cells. In this study, we developed a primary cultured organoid in 2D culture (2D organoid; 2DO) that maintained the clinical phenotypic heterogeneity of tumor samples. The parental tumor differentiation was preserved in our system, with gene expression similar to that in the parental tumor. In vitro drug sensitivity analysis can be conducted using the 2DO to reflect individual clinical courses. The 2DO developed in this study is an in vitro personal cancer model. This approach may be useful for producing clinical chemotherapy agents as personalized medicines.
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Free Research Field |
消化器癌
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Academic Significance and Societal Importance of the Research Achievements |
我々の樹立した初代培養細胞は基本的な培養方法を2次元培養としており(two-dimensional (2D) cultured organoid, 2DO)、非常に汎用性がある手法で、薬剤感受性試験を含む様々な研究手法において簡便に用いることができるマテリアルである。2DOを用いることで、より臨床の腫瘍に近い細胞挙動の解析が行え、多くのがん研究や個別化医療における有用なソースとなり得る。
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