2017 Fiscal Year Research-status Report
Elucidating the mechanism of mitochonria participation in the development of diabetic cystopathy. Is there a role of sirtuin 1 gene?
| Project/Area Number |
17K16793
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| Research Institution | Tottori University |
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Principal Investigator |
ツナピ パナイオタ 鳥取大学, 医学部附属病院, プロジェクト研究員 (50767431)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | diabetes / bladder / urological complications / sirtuin 1 |
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| Outline of Annual Research Achievements |
In the voiding behavior studies, urine production, micturition frequency, and single voided volume in the diabetic rats (no treatment +/- exercise protocol) were significantly larger than that in the control rats. Resveratrol treatment slightly improved the voiding behavior but the differences were not statistically significant compared to the diabetic group without treatment. Bladder weights were significantly higher in the diabetic animals compare to the Control animals. Resveratrol treatment significantly decreased the bladder weight compared to the non treated diabetic animals. Histological damages in the tissue of the bladder of the non-treated diabetic groups were prevented in both resveratrol treated groups (+/- exercise).
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Because of the high mortality in the diabetic groups, addition of experiments and animals was necessary. As a result, this delayed a little bit the schedule of our experiments.
Because of the extremely high cost of SRT1720, we replaced it with resveratrol, which is also an activator of situin 1 gene.
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| Strategy for Future Research Activity |
The animal groups will be created as described in the protocol. The only differentiation will be the replacement of SRT1720 by resveratrol.
Three days before the completion of the study voiding studies will be performed for all animals. On the completion of the metabolic cage period the animals will be subjected to Cystometrography . The bladders will be collected by all animals under anaesthesia.
The mitochondrial function will be investigated for all samples.
Oxidative stress parameters will be investigated in the bladder tissue by spectrophotometry and further their localization in the tissue by immunohistochemistry. Additionally apoptosis will be identified by TUNEL staining.
Histological evaluation will be performed under light microscope in hematoxylin-eosin staining.
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